phenol-stacked carbon nanotubes: a new approach to genomic dna isolation from plants
Authors
abstract
extraction of intact quality dna from plant tissues, especially those rich in secondary metabolites, is often challenging. literally, hundreds of different dna isolation protocols from various plant species have been published over the last decades. although many commercial dna isolation kits are convenient and designed to be safe, their cost and availability cause limitations in small molecular labs in many developing countries. in nearly all protocols and dna isolation kits, phenol and chloroform are used to precipitate various classes of impurities. however, phenol is partially soluble in water, resulting in the co-existence of proteins in upper (aqueous) phases. this phenomenon results in the contamination of the nucleic acids and low quality dna. nanotechnology advances have helped many areas of molecular biology such as the development of new diagnosis and purification kits. in this study, for the first time, we report a different approach to isolate dna from plants based on carbon nanotubes (cnts). the results show that the phenol reagent stack on cnts can effectively remove proteins, polysaccharides and other polyphenol constituents. the a260/a280nm absorbance ratios of isolated dna samples were 1.9 and 1.8 for chamomile and opium plants, respectively, indicating the high purity of the isolated dna. dna yield was more than two times the standard doyle and doyle method. furthermore, the isolated dna proved amenable to pcr amplification, using random amplified polymorphic dna (rapd) analysis.
similar resources
Phenol-stacked carbon nanotubes: A new approach to genomic DNA isolation from plants
Extraction of intact quality DNA from plant tissues, especially those rich in secondary metabolites, is often challenging. Literally, hundreds of different DNA isolation protocols from various plant species have been published over the last decades. Although many commercial DNA isolation kits are convenient and designed to be safe, their cost and availability cause limitations in small molecula...
full textPhenol-stacked carbon nanotubes: A new approach to genomic DNA isolation from plants
Extraction of intact quality DNA from plant tissues, especially those rich in secondary metabolites, is often challenging. Literally, hundreds of different DNA isolation protocols from various plant species have been published over the last decades. Although many commercial DNA isolation kits are convenient and designed to be safe, their cost and availability cause limitations in small molecula...
full texta new approach to credibility premium for zero-inflated poisson models for panel data
هدف اصلی از این تحقیق به دست آوردن و مقایسه حق بیمه باورمندی در مدل های شمارشی گزارش نشده برای داده های طولی می باشد. در این تحقیق حق بیمه های پبش گویی بر اساس توابع ضرر مربع خطا و نمایی محاسبه شده و با هم مقایسه می شود. تمایل به گرفتن پاداش و جایزه یکی از دلایل مهم برای گزارش ندادن تصادفات می باشد و افراد برای استفاده از تخفیف اغلب از گزارش تصادفات با هزینه پائین خودداری می کنند، در این تحقیق ...
15 صفحه اولSemiconducting properties of cup-stacked carbon nanotubes
The current–voltage characteristics of individual cup-stacked carbon nanotubes (CSCNTs) were investigated in situ inside the transmission electron microscope. Different from other quasi-1D carbon structures such as multi-walled carbon nanotubes, carbon nanofibers or graphitic fibers that normally behave as a metallic conductor of electrons, individual CSCNTs were found to exhibit unexpectedly s...
full textfrom linguistics to literature: a linguistic approach to the study of linguistic deviations in the turkish divan of shahriar
chapter i provides an overview of structural linguistics and touches upon the saussurean dichotomies with the final goal of exploring their relevance to the stylistic studies of literature. to provide evidence for the singificance of the study, chapter ii deals with the controversial issue of linguistics and literature, and presents opposing views which, at the same time, have been central to t...
15 صفحه اولAn efficient and simple CTAB based method for total genomic DNA isolation from low amounts of aquatic plants leaves with a high level of secondary metabolites
An efficient DNA isolation protocol specifically modified to get pure quality DNA required for molecular studieshas been reported in this paper. Some aquatic plants (Potamogeton spp., Ceratophyllum demersum and Myriophyllum spicatum) were used for the study. The protocol developed will be useful in getting high and pure DNA. Instead of using the available DNA extraction kits, this protocol can ...
full textMy Resources
Save resource for easier access later
Journal title:
molecular biology research communicationsPublisher: shiraz university press
ISSN 2322-181X
volume 3
issue 3 2014
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023